These data consist of raw 16S rRNA gene sequences for the bacterial communities in three upland Welsh river sites under different treatments. A mapping file with metadata for each sample is provided and a operational taxonomic unit (OTU) table. These sites were situated in three streams from the Llyn Brianne Stream Observatory, Powys, Wales, UK (52°08' N, 3°45' W). The catchments cover approximately 300 square kilometres of upland Wales in the upper Afon Tywi. These first to third order experimental streams rise in either rough, sheep-grazed moorland (named as L6 and L7) or plantations of Sitka spruce Picea sitchensis with lodgepole pine Pinus contorta (named as L3). Some reductions of forest cover have occurred in L3 with normal logging operations. A 24-hour experiment was conducted at the Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) cascading flumes at these streams during September 2014. Each flume consisted of 3 channels, each assigned a different treatment: control, sugar addition and peat addition. Sugar (sucrose) and peat were added to channels to represent a simple and complex form of dissolved organic carbon (DOC) respectively. Five biofilm samples were collected from random locations in each experimental channel. Samples were taken at 0.5, 3, 15 and 24 hours after the start of the experiment. Epilithon were taken from unglazed ceramic tiles that had been colonised by epilithon in the river. After amplification, the 16S rRNA fragments were sequenced on the Illumina MiSeq next generation sequencing platform. The main goal of this survey was to characterise bacterial diversity, the chemical and biological consequences of elevated DOC inputs, and to investigate the role of bacterial organisms in controlling organic carbon flux. Prof Andy Weightman and Dr Isabelle Durance were responsible for organising the experiments. Sampling was carried out by Dr. Isa-Rita Russo and a team of Post Doctoral Research Assistants (PDRA's)/students. The work was carried out under the Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/df829b9f-c4c5-4e53-9217-c9c1e5bd078d

This dataset consists of fish species, fish length and fish weight from samples taken from small and medium catchments across Wales. Samples were collected in the summers and autumns of 2012 and 2013 using electrofishing. All the collected individuals were counted and recorded to represent the fish diversity and abundance of each sampling site and date. The main goal of this survey was to relate fish production with a gradient of aquatic biodiversity associated with different environmental settings (land-use intensify, recovery from acidification). Dr Isabelle Durance was responsible for organising the surveys, Dr Hugh Feeley was in charge of collecting, sorting and measuring the fish samples. The work was carried out under the Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/57f95ff8-e1b0-41b9-866a-feb231aecb67

These data are for macroinvertebrate taxonomy and abundance of Welsh upland rivers. The sampling sites were located in small and medium catchments across Wales. Sampling took place in either spring 2013 for sites in the Wye catchment or during 2012 for the other catchments. At each sampling point, 2-minute kick-samples were taken from river riffles to represent macroinvertebrate composition. Samples were preserved in industrial methylated spirit on site. Samples were then sorted and identified in the laboratory. The main goal of this survey was to characterise a gradient of aquatic biodiversity associated with different environmental settings for example land-use intensify and recovery from acidification. Dr Isabelle Durance was the responsible of organising the surveys, Dr Hugh Feeley was in charge of collecting, processing and sorting the invertebrate samples. Kath Layer was commissioned by Cardiff University for the identification. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/8f7b2269-73b1-449f-8590-e36c7c850913

These data are suspended organic matter stocks of coarse and fine particulate organic matter in eight Welsh upland rivers with contrasting land-use, moorland and exotic conifer, in response to riparian deciduous leaf addition. Eight sampling reaches were chosen at two sites, Llyn Brianne (4 reaches) and Plynlimon (4 reaches). The experiment consisted of adding deciduous leaves to half of the reaches whilst the other half were maintained as a control (no addition of deciduous leaves). To characterise the suspended organic matter of the studied streams, water was filtered to collect monthly samples from December 2012 to January 2013 (before deciduous leaf addition) and from February to April 2013 (after deciduous leaf addition) in each sampling reach. The main goal of this survey was to examine how aquatic biodiversity and organic matter stocks respond to leaf addition in moorland and conifer forested rivers. Dr Isabelle Durance was responsible for organising the surveys, Marian Pye was in charge of collecting, processing and sorting the samples. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/37e7a1b3-0564-4c6f-84df-a9635edb57b4

The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274

These data are macroinvertebrate composition and size in eight Welsh upland rivers with contrasting land-use, moorland and exotic conifer, in response to riparian deciduous leaf addition. Eight sampling reaches were chosen at two sites, Llyn Brianne (4 reaches) and Plynlimon (4 reaches). The experiment consisted of adding deciduous leaves to half of the reaches whilst the other half were maintained as a control (no addition of deciduous leaves). To characterise the benthic macroinvertebrate communities of the studied streams, Surber net sampling was used to collect monthly samples during 2013 in January (before deciduous leaf addition) and from February to April (after deciduous leaf addition) in each sampling reach. Some of the collected individuals were used to characterise the invertebrate biomass of each reach. The main goal of this survey was to examine how aquatic biodiversity responds to leaf addition in moorland and conifer forested rivers. Dr Isabelle Durance was responsible for organising the surveys, Dr Hugh Feeley and Marian Pye, were in charge of collecting, processing and sorting the invertebrate samples. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/d07fe8a7-d4da-43e5-834d-e1b0f4cff0ac

A spatial approach was developed to interpret qualitatively expressed scenarios, and predict the probability and amount of change for 10 land-cover types across 127 sub-catchments in upland Wales. Existing data, which have a temporal coverage of 1998-2007, were used for the underpinning mapping, and fed into the tabular land cover change summary data. For each scenario, the maximum and minimum land-cover change was projected using rules based on current land cover, agricultural land quality, ownership type, and nature conservation status. For each combination, total land-cover change summaries have been created, which indicate how land cover within the 127 sub-catchments may respond to change in the future. This work was part of the Diversity in Upland River Ecosystem Service Sustainability (DURESS) project, NERC grant NE/J014826/1. Full details about this dataset can be found at https://doi.org/10.5285/0dd30cc6-d4fb-42f5-a5a4-954cf01a230b

This datasets contains Electrical Resistivity Tomography surveys taken in the Makutapora Basin, Central Tanzania, using an AGI SuperSting R8 (STING) resistivity meter. Survey geometry, parameters and coordinates are also included. Full details about this dataset can be found at https://doi.org/10.5285/1998da32-a978-41a4-8a66-81df1e625cca

These data consist of stream water chemistry for selected Welsh upland rivers. The sampling sites were located in sixty one small and medium catchments. Catchments were chosen from the Welsh Acid Water Surveys (WAWS) program (41 sites) and the Wye catchment (20 sites). Results for pH, alkalinity, conductivity and major cation and anion measurements are presented for the WAWS catchments. Results for pH, alkalinity, conductivity and major anion measurements are presented for the Wye catchment. Samples from the Wye catchment were collected in May 2012. Samples from the WAWS catchments were taken during the summer and autumn of 2012 and spring and summer of 2013. The data were collected to characterise water chemistry variation along a gradient of aquatic biodiversity associated with different environmental settings for example land-use intensify and recovery from acidification. Dr Isabelle Durance was responsible for organising the surveys, Dr Hugh Feeley was in charge of collecting and preserving the water samples. Analysis of the water samples was carried out at the Forest Research Laboratories. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/d329ca18-c3d8-49f0-b2fd-5243d76dc650

These data are macroinvertebrate composition and abundance in eight Welsh upland rivers with contrasting land-use, moorland and exotic conifer, in response to riparian deciduous leaf addition. Eight sampling reaches were chosen at two sites, Llyn Brianne (4 reaches) and Plynlimon (4 reaches). The experiment consisted of adding deciduous leaves to half of the reaches whilst the other half were maintained as control (no addition). Nylon mesh bags containing deciduous leaves were fixed in both experimental and control reaches to estimate leaf decomposition rates. The macroinvertebrate composition of the nylon mesh bags was surveyed in January 2013 and again in March 2013 in each sampling reach. The main goal of this survey was to examine how aquatic biodiversity responds to leaf addition in moorland and conifer forested rivers. Dr Isabelle Durance was responsible for organising the surveys, Dr Hugh Feeley, Dr Dan Perkins and Marian Pye were in charge of collecting, processing and sorting the invertebrate samples. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was a project funded by the Natural Environment Research Council (NERC) Biodiversity and Ecosystem Service Sustainability (BESS) programme Full details about this dataset can be found at https://doi.org/10.5285/3e6a9ffd-59f2-48ea-840b-1582dc6c1e3d